SISCAPA Assay / Training Kit
A lack of quantitative assays for most human proteins is a barrier to verification of the large number of protein biomarker candidates being produced in "-omics" experiments and also impedes basic research studies where relationships between protein abundance and biology are sought.
Stable Isotope Standards and Capture by Anti-Peptide Antibodies (SISCAPA) meets this need by combining peptide immunoaffinity enrichment with quantitative mass spectrometry (Anderson NL, Anderson NG, Haines LR, Hardie DB, Olafson RW, Pearson TW. in J Proteome Res. 2004 Mar-Apr;3(2):235-44.) To implement a SISCAPA assay, there is value to having a known standard that can be used both as a training tool for learning this technique and as a reference quality control (QC) assay for regular monitoring of system performance.
In collaboration with the Fred Hutchinson Cancer Research Center, Bio-Rad Laboratories has developed a SISCAPA training / QC kit. The kit enables researchers who are new to the SISCAPA technique to implement an assay in their lab and gain experience with the process. Based on an assay for a murine osteopontin (OPN) peptide in a human plasma matrix, the kit provides researchers with the reagents and information needed for carrying out an assay, including a detailed standard operating procedure, antibody, heavy and light peptides, magnetic beads, and other necessary buffers and reagents. Results will be comparable to expected values, making the kit a valuable resource for quality control of the SISCAPA process.
For more details about the development of this assay see the article by Whiteaker, Jeff R., Zhao, Lei, Anderson, Leigh, and Paulovich, Amanda G. in Molecular & Cellular Proteomics 9.1, (2010), 184 - 196.
If you would like to evaluate a kit, free of charge (while supplies last), please contact Tom Slyker at Tom_Slyker@Bio-Rad.com or 510-741-6747.
