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Agenda and Presentations

Proteomic Technologies Reagents Resource Workshop

Monday, December 12    Tuesday, December 13    Post-Workshop Meetings

Monday, December 12
8:00 a.m. - 8:30 a.m.

Welcome, Introductions, and Meeting Overview PDF File (Size: 11 KB)

Anna D. Barker, Ph.D.
National Cancer Institute

Leland H. Hartwell, Ph.D.
Fred Hutchinson Cancer Research Center

Meeting Co-Chairs:
Brian B. Haab, Ph.D.
Van Andel Research Institute

Amanda Paulovich, M.D., Ph.D.
Fred Hutchinson Cancer Research Center
8:30 a.m. - 9:00 a.m.

Session 1: Vision of a Shared Proteomics Reagents Resource

  • What are the needs of the scientific community?
  • What are the goals in building such a resource?

Chair:
N. Leigh Anderson, Ph.D.
Plasma Proteome Institute
9:00 a.m. - 10:00 a.m.

Session 2: New Opportunities To Advance Proteomic Technologies for Cancer Research

  • NCI’s initiatives in proteomic technology development
  • Perspectives on research resources – history and mechanisms
  • Emerging international initiatives for affinity capture methods and characterization
  • Establishing standards in biospecimen research and biobanking

Chair:
Gregory J. Downing, D.O., Ph.D.
National Cancer Institute

Adam Clark, Ph.D. PDF File (Size: 1 MB)
National Cancer Institute Protein Atlas

Mathias Uhlen, Ph.D. PDF File (Size: 2.2 MB)
KTH Biotechnology

Carolyn C. Compton, M.D., Ph.D.
National Cancer Institute
10:15 a.m. - 12 noon

Session 3: Proposed Models for an Antibody Reagent Resource PDF File (Size: 14 KB)

  • Strategies for creating and operating an effective antibody resource for the research community
  • Understanding best values for the research community\
  • Approaches for biological characterization
  • Requisite public-private partnership elements

Chair: N. Leigh Anderson, Ph.D.
Plasma Proteome Institute

Brian B. Haab, Ph.D.
Van Andel Research Institute

Panel Discussants:
Ed Harlow, Ph.D.
Harvard Medical School

Joshua LaBaer, M.D., Ph.D.
Harvard Institute of Proteomics
1:00 p.m. - 1:45 p.m.

Session 4: Applications of Affinity Capture Methods in Cancer Research PDF File (Size: 97 KB)

  • In what types of applications should affinity reagents be required to perform?
  • What types of quality control exist for these applications?

Chair: Amanda Paulovich, M.D., Ph.D.
Fred Hutchinson Cancer Research Center
1:45 p.m. - 2:30 p.m.

Session 5: Affinity Capture Reagents and Quality Control/Validation

  • Scientific validation: What is required to “prove” an antibody works?
  • What type(s) of validation exist for each type of reagent that it is binding to its intended target within an acceptable range of specificity and sensitivity?
  • How will new technologies be validated?
  • Should validation be done in a centralized facility or in a distributed fashion?
  • What level of quality control will be required for capture reagents available through the resource?
  • What standards commonly used can be adopted?
  • What new standards need to be created?

Chair: Brian B. Haab, Ph.D.
Van Andel Research Institute
3:15 p.m. - 4:30 p.m.

Session 6: Current Technologies – Overview of Poster Presentations

  • Companies and the research community are invited to present posters on their particular affinity capture technology to educate attendees on the state of the art.
  • Brief presentations from companies (3-5 minutes) detailing their ability to meet the user community’s needs.

Chair: Mathias Uhlen, Ph.D.
KTH Biotechnology

Posters:

 
6:15 p.m.

What does the future hold for affinity capture technologies in cancer research?

Speaker: Patrick O. Brown, M.D., Ph.D.
Stanford University School of Medicine

Tuesday, December 13
8:00 a.m. - 9:00 a.m.

Session 7: Target Selection PDF File (Size: 48 KB)

What peptide/protein targets should be chosen for the reagents resource?

  • What is the process by which they should be chosen?
  • What targets have already been chosen by other projects such as the Protein Atlas and what were the criteria?

Chair: N. Leigh Anderson, Ph.D.
Plasma Proteome Institute

9:00 a.m. - 10:00 a.m.

Session 8: Building a Database PDF File (Size: 196 KB)

  • Database of reagents: What characteristics should be catalogued?
  • Database of targets: What characteristics should be catalogued?
  • Who can read from/write to the database?
  • Database architecture: Centralized or decentralized?

Chair: Henning Hermjakob
EMBL-European Bioinformatics Institute
10:15 a.m. - 11:30 a.m. Session 9: Building a Shared Resource and Identifying Action Items PDF File (Size: 33 KB)
 
  • Feedback on resource reagent models.
  • Production: Who will produce reagents? How will producers be chosen?
  • Distribution: What are the guidelines for access to reagents?
  • Agree on action items for the short and long terms.

Chairs: Joshua LaBaer, M.D., Ph.D.
Harvard Institute of Proteomics

N. Leigh Anderson, Ph.D.
Plasma Proteome Institute

Brian B. Haab, Ph.D.
Van Andel Research Institute
11:30 a.m. - 12 noon

Wrap-up and Adjournment

  • Comments from Leland H. Hartwell, Ph.D.
  • Last thoughts from attendees
  • Next steps

Post-Workshop Meetings (optional)
12 noon - 2:00 p.m. Roundtable Discussion for Industry Representatives
NCI and SAIC (Science Applications International Corporation)-Frederick will be on hand to answer questions regarding the NCI’s initiatives and potential business opportunities.