CPTAC Develops Fit-for-Purpose Immuno-MRM Assay for FANCD2 Protein Modification Involved in DNA Damage
Fanconi anemia (FA) is a rare inherited disorder characterized by progressive bone marrow failure and an increased risk of developing certain types of cancer. The FA pathway consists of a network of 21 proteins that is specialized for repairing DNA inter-strand cross-links. Identification of monoubiquitylation (or similar) defects in this network provides an opportunity for therapeutic targeting with a high-throughput, quantitative mechanism. CPTAC investigator Dr. Amanda Paulovich and her research team at the Fred Hutchinson Cancer Research Center demonstrated the utility of an immuno-multiple reaction monitoring (iMRM) assay to quantify the unmodified and monoubiquitinated protein isoforms and peptides unique to FANCD2.